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 Glenn van AsSpectatorNeat study…….of course it was in enamel but my big big question. What were the settings. Glenn 
 AnonymousGuestGlenn, 
 With the hydrokinetics it doesn’t matter what settings you use đActually, I emailed the author and will let you know if I get a response. Happy New Year! 
 Glenn van AsSpectatorOh darn it all ROn ……….now you tell me HK really does exist and I didnt include it in the text chapter……. Oh man guess I better go revise it. JUST KIDDING Glenn 
 AnonymousGuestGlenn, Dr. Usumez was kind enough to send the whole study. Bond strengths of porcelain laminate veneers to tooth surfaces prepared 
 with acid and Er,Cr:YSGG laser etching
 Aslihan Usumez, DDS, PHD,a and Filiz Aykent, DDS, PHDb
 Faculty of Dentistry, Selcuk University, Konya, Turkey
 Statement of problem. The erbium, chromium: yttrium, scandium, gallium, garnet (Er,Cr:YSGG) hydrokinetic
 laser system has been successful in the ablation of dental tissues. It has been reported that this system is also
 useful for preparing tooth surfaces for adhesion, but results to date have been controversial.
 Purpose. This in vitro study evaluated the bond strengths of porcelain laminate veneers to tooth surfaces after
 etching with acid and Er,Cr:YSGG laser conditioning.
 Material and method. Forty extracted caries- and restoration-free human maxillary central incisors were used.
 The teeth were sectioned 2 mm below the cementoenamel junction. The crowns were embedded in autopolymerizing
 acrylic resin with the labial surfaces facing up. The labial surfaces were prepared with .05 mm reduction
 to receive porcelain veneers. The teeth were divided into 4 groups of 10 specimens. Thirty specimens received 1
 of the following surface treatments before the bonding of IPS Empress 2 laminate veneers: (1) laser radiation
 from an Er,Cr:YSGG laser unit; (2) 37% orthophosphoric acid; and (3) 10% maleic acid. Ten specimens received
 no surface treatment and served as the control group. The veneers were bonded with dual-polymerizing resin,
 Variolink II. One microtensile specimen from each of the cervical and incisal thirds measuring 1.2 1.2 mm was
 prepared with a slow-speed diamond saw sectioning machine with a diamond-rim blade. These specimens were
 attached to opposing arms of the microtensile testing device with cyanoacrylate adhesive and fractured under
 tension at a crosshead speed of 1 mm/min, and the maximum load at fracture (Kg) was recorded. The data were
 analyzed with a 2-way analysis of variance and Tukey HSD tests (.05).
 Results. No statistically significant differences were found among the bond strengths of veneers bonded to
 tooth surfaces etched with Er,Cr:YSGG laser (12.1 4.4 MPa), 37% orthophosphoric acid (13 6.5 MPa), and
 10% maleic acid (10.6 5.6 MPa). The control group demonstrated the lowest bond strength values in all test
 groups. Statistically significant differences were found between the bond strengths of cervical and incisal sections
 (P.001).
 Conclusion. In vitro microtensile bond strengths of porcelain laminate veneers bonded to tooth surfaces that
 were laser-etched showed results similar to orthophosphoric acid or maleic acid etched tooth surfaces.
 (J Prosthet Dent 2003;90:24-30.)
 CLINICAL IMPLICATIONS
 This in vitro study reported no difference in microtensile bond strengths of porcelain veneers
 bonded to tooth surfaces that were etched with an Er,Cr:YSGG laser, 37% orthophosphoric acid,
 or 10% maleic acid.
 Patient demand for the treatment of unesthetic anterior
 teeth has grown. For many years the most predictable
 and durable esthetic correction of anterior teeth has
 been achieved by the preparation of complete crowns.1
 However, this approach is undoubtedly the most invasive
 with the removal of substantial amounts of sound
 tooth substance with possible adverse effects on adjacent
 pulp and periodontal tissues.2,3
 Calamia4 described the clinical and laboratory procedures
 for bonding porcelain laminate veneers to acid
 etched enamel. The popularity of porcelain laminate veneers
 has increased since their introduction because
 tooth preparation is conservative and the restorations
 are esthetic.5 However, an in vitro study has described
 some disadvantages such as marginal adaptation and related
 bonding problems.6
 Traditionally, etching the enamel surface with orthophosphoric
 acid, a concept first proposed by Buonocore,
 7 has been commonly used to increase the bond
 strength between the composite and enamel. The technique
 of etching with orthophosphoric acid is used to create
 an irregular surface of enamel. This allows an increase in
 the prepared surface area available for the retention of the
 composite and an improvement in the marginal adaptation
 of laminate veneers.8 The retentive characteristics of acidconditioned
 enamel surfaces depend on the type of acid,
 etching time, and chemical composition of enamel.9
 aAssistant Professor, Department of Prosthodontics.
 bAssociate Professor, Department of Prosthodontics.
 24 THE JOURNAL OF PROSTHETIC DENTISTRY VOLUME 90 NUMBER 1
 Three types of etching patterns have been described
 by Silverstone et al10 after exposure of the enamel prisms
 to etching solutions: type I, preferential removal of
 prism core material, leaving the periphery intact; type II,
 preferential removal of periphery core material, leaving
 the prism core relatively unaffected; and type III, a more
 random etching pattern in which adjacent areas of the
 tooth surface correspond to types I and II, mixed with
 regions in which the pattern could not be related to
 prism structure. Morphologic information obtained by
 scanning electron microscopy indicated that the surface
 structure resulting from etching with 35% orthophosphoric
 acid and 10% maleic acid is similar.11,12
 Laser devices have been used in dentistry for soft
 tissue surgery, root end sealing and sterilization, and for
 altering enamel and dentin surfaces to increase resistance
 to decay or to facilitate the bonding of composites.13,14
 Laser etching may be an alternative to acid etching of
 enamel and dentin. Laser etching is painless and does
 not involve either vibration or heat, making this treatment
 attractive.15 Furthermore, laser etching of enamel
 or dentin has been reported to yield an anfractuous surface
 (fractured and uneven) and open dentin tubules,
 both apparently ideal for adhesion.15 The surface produced
 by laser etching is also acid-resistant because laser
 radiation of dental hard tissues modifies the calcium-tophosphorus
 ratio, reduces the carbonate-to-phosphate
 ratio, and leads to the formation of more stable and less
 acid-soluble compounds, thus reducing susceptibility to
 acid attack and caries.16
 The ability of erbium:yttrium aluminum garnet (Er:
 YAG) lasers to cut dental biocalcified tissue effectively
 has been demonstrated.15 Furthermore, the cutting ef-
 ficacy is improved when the tooth surfaces are flooded
 with a water layer.15,17,18 The Er,Cr:YSGG pulsed-wave
 laser, when used with an air-water spray, has been shown
 to cut enamel, dentin, cementum, and bone efficiently
 and cleanly.19,20 The Er,Cr:YSGG laser produces microexplosions
 during tissue ablation, resulting in macroscopic
 and microscopic irregularities.21 The Er,Cr:
 YSGG laser initially causes vaporization of water and
 other hydrated organic components of the tissue.21 On
 vaporization, the internal pressure builds within the tissue
 until the explosive destruction of inorganic substance
 occurs before the melting point is reached.21
 The quality of the bond obtained by laser etching of
 enamel relates to the energy densities of the device.22
 With low energy densities, the surface is largely unaffected
 by laser pulses and retention is poor. At intermediate
 exposures surface roughening occurs.22 At high
 energy densities, the enamel is fused and this thin layer
 of fused enamel becomes the weakest link in the chain of
 adhesion.22
 Laser-induced physical changes include melting and
 recrystallization with the formation of numerous pores
 and small, bubble-like inclusions. These profiles have
 been shown by some studies in CO2 laser23 and Nd:
 YAG laser.24,25 In contrast, no melting or recrystallization
 was observed with Er,Cr:YSGG hydrokinetic system.
 19,26
 The purpose of this study was to determine the microtensile
 bond strengths of porcelain laminate veneers
 to acid-etched and Er,Cr:YSGG laser treated enamel,
 with an unetched group serving as the control. The
 enamel morphologic structure after laser etching and
 acid etching was also investigated with scanning electron
 microscopy (SEM). The hypothesis tested was that the
 microtensile bond strength obtained after Er,Cr:YSGG
 laser etching of enamel is similar to that obtained after
 acid etching.
 MATERIAL AND METHODS
 Forty extracted human maxillary central incisors with
 10 mm anatomic crown length and 8 mm mesiodistal
 width were selected. Each tooth was free of dental caries
 and restoration. The teeth were cleaned and stored in
 saline solution at room temperature immediately after
 extraction.
 The teeth were sectioned 2 mm below the cementoenamel
 junction with a slow-speed diamond saw sectioning
 machine (Isomet; Buehler Ltd, Lake Bluff, Ill), and
 the crowns were embedded in autopolymerizing acrylic
 resin (Meliodent; Bayer Dental Ltd, Newbury, UK)
 with the labial surfaces facing up.
 Tooth preparation
 The facial surfaces of the teeth were prepared to accommodate
 veneers of equal thickness. A 0.5-mm facial
 reduction was performed with a chamfered cervical finish
 line and incisal bevel preparation. Self-limiting
 depth-cutting disks of 0.5 mm (834-31-021; Gebr.
 Brasseler, Lemgo, Germany) were used to define the
 Fig. 1. Completed veneer preparation.
 USUMEZ AND AYKENT THE JOURNAL OF PROSTHETIC DENTISTRY
 JULY 2003 25
 depth of the cuts, and then 1.4-mm chamfer diamond
 burs (6844-314-014; Gebr. Brasseler) were selected to
 refine the preparation. All tooth preparations were completed
 without sharp line angles (Fig 1).
 Impression making and master die fabrication
 Impressions of the 40 prepared teeth were made with
 polyvinylsiloxane impression material (Permagum; 3M
 ESPE AG, Seefeld, Germany). The impressions were
 poured with a vacuum-mixed polyurethane die material
 (Alpha Die MF; Schušltz-Dental GmbH, Rosbach, Germany)
 according to the manufacturersâ instructions with
 respect to water/power ratio and mixing time. Dies
 were recovered from the impressions, and 2 layers of die
 spacer (Cement Spacer; Kerr Dental, Orange, Calif)
 were painted 0.5 mm short of the finish lines of the
 preparations.
 Ceramic veneer fabrication
 The veneers were waxed (Yeti Dental produkte;
 GmbH, Engen, Germany), sprued, and then pressed
 after investment. All procedures were performed with
 IPS Empress 2 materials (Ivoclar, Schaan, Liechtenstein),
 following the manufacturerâs recommendations.
 After divestment, the ceramic veneers were finished with
 diamond burs (863-204-016; Gebr. Brasseler) and
 glazed.
 Surface treatment
 The 40 prepared teeth were randomly assigned to 4
 groups of 10 specimens (n 10). Each of 3 groups was
 subjected to a different etching technique (Table I). Ten
 specimens received no surface treatment and served as
 the control group.
 Laser treatment
 An Er,Cr:YSGG hydrokinetic dental laser (Millennium;
 Biolase Technology, Inc., San Clemente, Calif) was
 used for laser etching. This hard- and soft-tissue laser
 creates laser-energized, atomized water droplets that act
 as cutting particles. Laser energy is delivered through a
 fiberoptic system to a sapphire tip terminal 6 mm long
 and 600 m in diameter, bathed in an adjustable air and
 water vapor. It operates at a wavelength of 2.78 m;
 pulse duration of 140 microseconds with a repetition
 rate of 20 Hz. Average power output can be varied from
 0 to 6W, depending on the tissue to be cut. The energy
 and power densities were (5.6 J/cm2) and (111 W/cm2
 at 2W), respectively, and were calculated by the manufacturer
 of the laser unit for the used power adjustment.
 The air and water spray of the hand-piece was adjusted
 to the â30â scale of the laser unit. The beam was aligned
 perpendicular to the enamel at 1 mm distance and was
 moved in a sweeping fashion by hand during an exposure
 period of 15 seconds over the entire area. The irradiated
 specimen was dried with an oil-free air source for
 15 seconds.
 Bonding ceramic veneers
 The ceramic veneers were treated with fluoridic acid
 (Ceramic Etchant; Ceramco, Burlington, NJ) for 1
 minute and neutralized (Ceramic Etchant Neutralizer;
 Ceramco) in accordance with the manufacturerâs instructions.
 Silane (Monobond S; Ivoclar) was first applied
 with a brush to the ceramic veneers for 60 seconds,
 and then a bonding agent (Heliobond; Ivoclar) was applied.
 After the teeth were etched, primer (Syntac Primer;
 Ivoclar) was applied to the tooth surface for 15 seconds,
 adhesive (Syntac Adhesive; Ivoclar) for 10
 seconds, and then a bonding agent (Heliobond; Ivoclar)
 with a brush.
 Cement (Variolink II; Vivadent, Ivoclar), comprising
 a combination of 25% Variolink yellow base, 25% Variolink
 white base, and 50% catalyst was hand-mixed following
 the manufacturerâs directions, and applied to
 both prepared teeth and the ceramic veneers. The ceramic
 veneers were placed on the prepared teeth with
 light finger pressure,27 and excess cement was removed
 with an explorer. Photo polymerization was performed
 with the light-polymerizing unit (Hilux 350; Express
 Dental Products, Toronto, Canada) at 350 mW/cm2
 (with a light tip to specimen distance of 0 mm) for 40
 seconds for incisal, mesial, and distal surfaces.
 Specimen preparation
 After cementation, specimens were stored in distilled
 water for 24 hours. Acrylic resin blocks were mounted in
 a slow-speed diamond saw sectioning machine (Isomet)
 with a diamond-rim blade.
 Two saw cuts were made parallel to the long axis of
 the tooth, and subsequently 4 saw cuts were made perpendicular
 to the long axis. This produced 2 I-shaped
 Table I. Materials used for surface conditioning
 Material Used
 Time of
 etching Brand Manufacturer
 37% orthophosphoric acid 15 s Bisco Bisco Inc, Schaumburg, Ill
 10% maleic acid 15 s Scotchbond Multi-Purpose 3M, St. Paul, Minn
 Er;Cr;YSGG hydrokinetic laser system 15 s Millennium Biolase Tech Inc, San Clemente, Calif
 THE JOURNAL OF PROSTHETIC DENTISTRY USUMEZ AND AYKENT
 26 VOLUME 90 NUMBER 1
 specimens, 1 from the incisal portion, and the other
 from the cervical (Fig. 2, A). The porcelain bonded to
 the facial enamel surface was divided into an array of
 1.2 1.2 5-mm beams (Fig. 2, B), with the top half
 consisting of porcelain and the bonding agent, and the
 bottom half consisting of enamel and dentin.28 Each
 specimen was tested individually.29
 Cyanoacrylate adhesive (Zapit; Dental Ventures of
 America, Corona, Calif) was used to attach the microtensile
 specimens to opposing arms of the microtensile
 testing device (Harvard Apparatus Co. Inc., Dover,
 Mass). The mounting adhesive was applied sparingly to
 the edges of each specimen. The specimen was fractured
 under tension at a crosshead speed of 1 mm/min, and
 the maximum load at fracture (Kg) was recorded. Preparation
 of all specimens and completion of the testing
 were done by the same operator.
 Fracture analysis
 After the specimen was tested and removed from the
 testing apparatus, the fracture sites were observed with a
 stereomicroscope (SZTP; Olympus, Tokyo, Japan) at
 original magnification 22 to identify the mode of failure.
 The fractured surface was classified according to 1
 of 3 types: (1) adhesive failure between the bonding
 resin and the enamel/dentin; (2) cohesive failure in the
 bonding resin; and (3) cohesive failure in the enamel/
 dentin.
 Statistical analysis
 The ultimate stress (MPa) of the porcelain-enamel/
 dentin bonds were calculated as follows:30
 Stress
 Failure Load (Kg)
 Surface area (mm2) 9.8
 The results of testing were entered into a spreadsheet
 (Excel; Microsoft, Seattle, Wash) for calculation of descriptive
 statistics. The obtained data were analyzed by
 2-way analysis of variance and then Tukey HSD tests
 (SPSS/PC, Vers.10.0; SPSS, Chicago, Ill) for pairwise
 comparisons among groups (.05).
 RESULTS
 Microtensile bond strengths
 The 2-way analysis of variance test indicated that tensile
 bond strength was significantly affected by position
 (cervical or incisal) (P.001) and treatment (acid or
 laser) (P.001), and there was no significant interaction
 between the 2 factors (P.05). Because there was no
 significant interaction, all data in each group were
 pooled. When the cervical and incisal data were pooled
 to investigate the effect of a particular surface treatment
 on bond strength, no statistically significant differences
 were found between the bond strength values of veneers
 bonded to 37% orthophosphoric acid (group B) and
 Er,Cr:YSGG laser-etched tooth surfaces (group A).
 Again, no statistically significant differences were found
 between the bond strengths of veneers bonded to 37%
 orthophosphoric (group B) and 10% maleic acid (group
 C) etched tooth surfaces. Statistically significant differences
 were found between the laser etched surfaces
 (group A) and the control (group D) (P.05). There
 were statistically significant differences between the orthophosphoric
 acid etched surfaces (group B) and the
 control (group D) (P.01). Additionally, no statistically
 significant differences were observed between laser
 etched and maleic acid etched tooth surfaces (Table II).
 Mean bond strength values for different treatment
 groups were calculated together with standard deviations
 (Fig. 3). The mean bond strength of group B was
 higher than the laser-treated group (group A) in the
 incisal sections, but in the cervical sections group A was
 higher. The control group (group D) demonstrated the
 lowest bond strength values in all test groups (Table
 III).
 Fig. 2. A, Sections on tooth for specimen preparation (shaded
 area represents section prepared from tooth specimen).
 B, Schematic demonstration of specimen.
 Table II. Microtensile bond strengths (MPa) statistical
 comparison
 Groups X SD
 Tukey
 grouping*
 Group A (Laser) 12.1 4.4 A
 Group B
 (Orthophosphoric acid)
 13.0 6.5 A
 Group C (Maleic acid) 10.6 5.6 A
 Group D (Control) 7.7 3.1 B
 X, Mean; SD, standard deviation.
 *Groups with different letters were statistically significantly different.
 USUMEZ AND AYKENT THE JOURNAL OF PROSTHETIC DENTISTRY
 JULY 2003 27
 Fracture patterns
 In the laser-treated group (group A), most failures
 (17 of 20) were adhesive in nature at the bonding resin/
 enamel interface, and 2 specimens showed cohesive failure
 in the bonding resin. Only 1 specimen showed cohesive
 failure within the enamel. In the group etched
 with orthophosphoric acid (group B), most failures (19
 of 20) were adhesive in nature at the bonding resin/
 enamel interface. One specimen showed cohesive failure
 within the enamel. The specimens in the group etched
 with maleic acid (group C) and in the control group
 (group D) showed adhesive fracture at the resin/enamel
 interface.
 Scanning electron microscopy
 SEM photographs of 37% orthophosphoric acid, 10%
 maleic acid, and Er,Cr:YSGG hydrokinetic laser-treated
 enamel are shown in Figure 4. The enamel surface
 etched with 2 acid solutions and a laser system showed
 different results according to Silverstoneâs10 etching
 patterns. The 37% orthophosphoric acid removed the
 periphery core material but left the prism core relatively
 unaffected (type II), producing a very rough enamel
 surface. The 10% maleic acid treatment resulted in preferential
 removal of prism core material and left the periphery
 intact (type I). Er,Cr:YSGG hydrokinetic lasertreated
 enamel showed a more random etching pattern
 in which adjacent areas of tooth surface correspond to
 types I and II, mixed with regions where the pattern
 could not be related to prism structure. There was no
 recrystallization or melting observed.
 DISCUSSION
 The results obtained support the research hypothesis
 of an expected similar adhesive force after laser treatment.
 This result is in accordance with the study of
 Usumez et al26 in which they compared these methods
 for bonding orthodontic brackets to enamel surfaces.
 On the other hand, the results of this study disagree with
 the results from other studies.14,23-25 These differences
 may be related to the different type of laser used, duration
 of exposure, and energy applied to the surface.
 Laser etching may have some advantages, but 1major
 limitation of lasers for dental application includes cost of
 laser units. They are still too expensive to be cost effective.
 This study also compared the microtensile bond
 strengths of specimens in the 10% maleic acid and 37%
 orthophosphoric acid etched groups. The results have
 indicated that there were no significant differences in
 microtensile bond strengths between the 2 groups. The
 results of this study are in agreement with the works of
 Goes et al11 and Hermsen and Vrijhoef.12
 For microtensile testing, the tensile bond strength is
 dependent on the area of the bonded surface.28 In this
 study, failures occurred mostly at the bonding resin/
 enamel interface and did not involve the enamel or ceramic
 except for the 2 specimens which showed cohesive
 Fig. 3. Mean microtensile bond strength values of test groups; groups with different letters are statistically significantly different.
 Table III. Microtensile bond strengths of cervical and
 incisal specimens (MPa)
 X SD
 Group A (laser) Cervical 10.6 4.1
 Incisal 13.5 4.2
 Group B
 (orthophosphoric) Cervical 8.3 3.7
 Incisal 17.7 5.2
 Group C (maleic) Cervical 7.6 3.3
 Incisal 13.5 6.0
 Group D (control) Cervical 5.7 2.5
 Incisal 9.7 2.5
 X, Mean; SD, standard deviation.
 THE JOURNAL OF PROSTHETIC DENTISTRY USUMEZ AND AYKENT
 28 VOLUME 90 NUMBER 1
 failure within the enamel. Microtensile testing should
 more closely approximate clinical applications.28 However,
 microcracks and other defects can possibly occur
 during the production of specimens with a slow-speed
 diamond saw sectioning machine, which may cause premature
 failure of the bond. Therefore the specimens
 must be prepared carefully.29
 Laser-treated enamel demonstrated strong bonding
 to the porcelain laminate veneers. The highest microtensile
 bond strength was achieved with 37% orthophosphoric
 acid for the incisal sections while the highest
 mean bond strength was achieved with laser treatment
 for the cervical sections. It is believed that these differences
 are due to exposure of the dentin layer in the
 cervical portions of specimen because of decreased
 thickness of enamel in this region. Visuri et al15 suggested
 that the greater presence of peritubular dentin,
 which has a greater mineral content than intertubular
 dentin, may result in better bonding to the dentin. In
 their study they obtained higher shear bond strength of
 composite when it was bonded to Er:YAG laser-prepared
 dentin compared with acid-etched dentin. Another
 difference between acid etchant and laser actions
 related to dentin is their effect on the structure of dentin
 tubules. When an acid etchant is applied, the peritubular
 dentin is preferentially etched, resulting in funnelshaped
 openings to the tubules. This structure may contribute
 with polymerization shrinkage to pull the tags
 away from the walls. Laser irradiation produces no demineralization
 of peritubular dentin and the dentinal tubules
 remain open with no widening.21 This effect may
 have contributed to microtensile bond strengths of cervical
 sections where dentinal exposures were present.
 Sources for the large deviations found in this study include
 variations in enamel structure, storage effects, age,
 condition of individual teeth, variations in enamel
 depth, and nonhomogenous laser treatment of surfaces.
 CONCLUSIONS
 Within the limitations of this study, 37% orthophosphoric
 acid (13.0 MPa)â and 10% maleic acid (10.6
 MPa)âtreated enamel surfaces showed statistically similar
 bond strength values. Porcelain laminate veneers
 demonstrated the highest bond strengths to 37% orthophosphoric
 acid-etched (13.0 MPa) and
 Er,Cr:YSGG hydrokinetic laser system-conditioned
 tooth surfaces (12.1 MPa). The differences were not
 statistically different.
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 JULY 2003 29
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 Reprint requests to:
 DR ASLIHAN USUMEZ
 SELCUK UNIVERSITY, FACULTY OF DENTISTRY
 DEPARTMENT OF PROSTHODONTICS
 CAMPUS/KONYA
 TURKEY
 FAX: 90-332-241-0062
 E-MAIL: asli_u@hotmail.com
 Copyright © 2003 by The Editorial Council of The Journal of Prosthetic
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 THE JOURNAL OF PROSTHETIC DENTISTRY USUMEZ AND AYKENT
 30 VOLUME 90 NUMBER 1
 2thlaserSpectatorNice Ron, 
 Here’s the latest from UCLA….Presented at the I3180 Bond Strength of Ceramic to Er,Cr:YSGG Laser Prepared Teeth 
 E.M. CHUNG1, E. SUNG1, A.A. CAPUTO1, M. COLONNA2, and I. RIZOIU2, 1 UCLA School of Dentistry, Los Angeles, CA, USA, 2 Biolase Technology Inc, San Clemente, CA, USA
 Objectives: A previous study has shown that composite resin bond strength to primary dentin prepared with an Er,Cr:YSGG laser was comparable or higher than to carbide bur prepared dentin. The purpose of this study was to compare the shear bond strength of bonded ceramic restorations to Er,Cr:YSGG laser prepared and conventionally prepared teeth. Methods: Ten surfaces were prepared into dentin of extracted human molars using each of the following: a) Er,Cr:YSGG hydrokinetic laser (Biolase Technology Inc), b) medium coarse parallel diamond bur (Brassler) with a high speed handpiece (Midwest). Ceramic discs (IPS Empress, Ivoiclar), 4.2 mm in diameter and 2 mm thick, were cemented onto the dentinal surfaces with composite resin cement (RelyX Unicem Aplicap, 3M EPSE). Shear bond strength tests were performed using an Instron test machine. After testing, the failure surfaces of the teeth were examined under 20X magnification. The bond strength data were examined statistically using ANOVA and t-test. Results: The mean values of the shear bond strength of the laser prepared surfaces were 8.23 ± 1.92 MPa and 4.88 ± 1.02 MPa for the conventional bur prepared surfaces. Statistical analysis revealed significant differences between the two groups (p.<0.05). Failure locations varied between the dentin-cement interface and ceramic interface. No predominant location patterns were observed between the laser and bur prepared teeth Conclusions: Higher bond strengths were seen with the laser prepared teeth. These higher values may be due to micromechanical retention or increased surface energy. There was more variability with the laser prepared teeth, indicating the need for a finishing surface treatment with the laser.Seq #339 – Cements: Dentin and Ceramic Bonding 
 10:15 AM-11:30 AM, Saturday, 13 March 2004 Hawaii Convention Center Exhibit Hall 1-2
 ADR meeting in Hawaii in March…We will continue to do more studies as time permits, and Eric and I discuss this study a bit more. Mark 
 AnonymousGuestQUOTEQuote: from 2thlaser on 1:04 pm on May 6, 2004
 Conclusions: Higher bond strengths were seen with the laser prepared teeth. These higher values may be due to micromechanical retention or increased surface energy. There was more variability with the laser prepared teeth, indicating the need for a finishing surface treatment with the laser.Mark Mark, the finishing surface treatment refers to a very defocused laser application to remove the byproducts of abaltion (like Graeme recommends), correct? As I don’t use Relyx, were the teeth etched? Thanks for keeping us up to date with the post, 
 Glenn van AsSpectatorWhat settings were used……….what wattage. What tip. What water concentration. What air concentration. What was the length of time used for the laser. How defocussed. Was the enamel scraped afterwards. Just other questions I had. The information out there on bonding with lasers is fraught with all kinds of mistakes. There are some studies showing way lower bond strength (particularly class V restorations) and others showing equal bond strength. Rare has it been shown to have higher bond strength. Neat stuff though , but be very careful suggesting that the laser will increase bond strength as there are many many variables to the equation including what I asked about above. Glenn 
 2thlaserSpectatorQUOTEQuote: from Glenn van As on 6:46 am on May 7, 2004
 What settings were used……….what wattage.What tip. What water concentration. What air concentration. What was the length of time used for the laser. How defocussed. Was the enamel scraped afterwards. Just other questions I had. Â The information out there on bonding with lasers is fraught with all kinds of mistakes. Â There are some studies showing way lower bond strength (particularly class V restorations) and others showing equal bond strength. Rare has it been shown to have higher bond strength. Neat stuff though , but be very careful suggesting that the laser will increase bond strength as there are many many variables to the equation including what I asked about above. Glenn Glenn, 
 I can appreciate where you are coming from however, until you understand how a crown is prepared with the laser, it’s hard to describe. No contact is made on the tooth. The power settings were my normal settings of 5.5W, 60/30 air/water, and a G-4 tip. The time it took was about 4-6 minutes per tooth to preapare with the laser, mostly in a defocussed mode approximately 3mm from the tooth. When I prepare a tooth for a crown, it’s NOT perpendicular to the tooth, rather more parallel to the surface I am preparing. I prepared all the specimens myself, both with the laser and the high speed specimens. All UCLA, and Eric did was to test the shear strength, and the conclusions you have read. There may be many variables as you stated, but I think you ought to be careful in suggesting that the data might suggest otherwise. This was a VERY controlled study, mainly due to my inquisitive nature on how strong a surface I am creating with the laser for cementation vs. a drilled prepared surface. Now, not being a scientist, but a inquisitive dentist, I tried to take the variables out as much as I could. Oh, and to answer your last question, the surface was not “scraped”. Just a pure lasered surface for cementation, and then shear strength testing. Hope that helps you. I appreciate your questions….!Mark 
 jetsfanSpectatorMark . For all of those old amalgams we remove with burs and finish off with the laser, perhaps you can quantify the benefit of actually using the laser, i.e, is the shear bond strength greater on a tooth that is just finished off with the laser in a defocused mode, than the tooth bonded without the laser treatment. Robert 
 AnonymousGuestMark, I think what Glenn and I were trying to get at with the questions is that if more details were available then your study could be compared ‘apples to apples’ with some of the other studies. Hopefully it could be discovered what was or wasn’t done in some of the other studies that showed less than stellar results. I take it no scraping because you felt all the byproducts of ablation were removed by using the defocused mode, correct? 
 2thlaserSpectatorCorrect. 
 Glenn van AsSpectatorHey Mark…….dont get me wrong. I admire your tenacity for the crown prep and also for doing these studies. Kudos to you. I am just being the devils advocate and telling you that even in Hibst review of hard tissue lasers he mentions that there are some diagramatically opposed viewpoints on the lasers effect on bond strength. I can give you quotes from my soon to be finished chapter (finally) but suffice it to say that if you look in the research , some of the bonding studies show lasers to be pretty abysmal with respect to bond strength. I have seen some fascinating stuff on bone where there is an “ablation “layer in the SEM when using the laser in bone. THis leaves a layer which even 18 months after the intial cuts in Rats (Aoki is the author) there still was the ablation layer visible in the histology on rats. What does this have to do with bonding? Well I sincerely believe that in many of the studies that a “ablation layer” was left behind particularly if there was no etch or Milicich scraping of the enamel after the laser was used. You know how I feel about the cutting effects of the Er,Cr:YSGG vs the Er:YAG and the similarities. I am proud of you for doing the research and am glad to see an article showing higher bond strengths. All I was saying is to be careful when you evaluate the other studies that have been done on hard tissue lasers and bonding or microleakage (Class V) as there are alot of studies showing worse bond strengths or greater leakage with the laser……. What did they do differently? Interesting debate isnt it? I still believe that with the proper technique, settings (not to high), scraping the enamel, and also etching that our bond strengths will be higher…….. I willl post one case before I turn in for the night……. check out what the scope showed. Grin Glenn PS I have the utmost respect for what you are doing, I am on your side full bore…….I think you have done more for my education in lasers than anyone perhaps except for Bob Gregg. Just keep that in mind……….ok!! Grin Glenn 
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