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    : Aust Endod J. 2004 Apr;30(1):20-2.  Related Articles, Links  

    Evaluation of the antimicrobial effect of Er:YAG laser irradiation versus 1% sodium hypochlorite irrigation for root canal disinfection.

    Perin FM, Franca SC, Silva-Sousa YT, Alfredo E, Saquy PC, Estrela C, Sousa-Neto MD.

    Faculty of Dentistry, Universidade de Ribeirao Preto, Ribeirao Preto, Sao Paulo, Brazil.

    This laboratory study evaluated Er:YAG laser antibacterial action in infected root canals. Forty-eight maxillary central incisors were used. After canal preparation, the teeth were autoclaved and divided into four groups: (1) non-treated teeth (control group); (2) teeth treated with NaOCl; (3) teeth irradiated with Er:YAG laser (7 Hz, 100 mJ, 80 pulses/canal, 11 sec) to the working length; (4) teeth irradiated similarly to, but 3 mm short, of the apex. The root canals from Groups 2, 3 and 4 were inoculated with 4 bacteria: Bacillus subtillus, Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus, together with Candida albicans, and maintained for 24 h at 37 degrees C. All suspensions were adjusted to tube 2 of the MacFarland scale. The intracanal material was then collected with sterile paper points, which were placed in the canals for 5 min and then immersed in 5 ml of BHI medium. This was then seeded onto agar and stained by Gram’s method. The NaOCl solutions and the Er:YAG laser irradiation to working length were effective against all five micro-organisms; however, 70% of the specimens irradiated 3 mm short of the apex remained infected.

    PMID: 15116905 [PubMed – indexed for MEDLINE]

    #9730 Reply

    Robert Gregg DDS

    ……..70% of the specimens irradiated 3 mm short of the apex remained infected.

    That’s not good.

    #9729 Reply

    Lee Allen

    Does anyone use this technique of 80 pulses at the apical foramen?

    Seems the Biolase technique uses 20 Hz and a withdrawal motion, but has it been studied like this?


    #9728 Reply


    I wonder why the study only use 1% sodium hypochlorite irrigation instead of the standard full strength? Wouldn’t it make more sense to compare the laser to the % now considered the standard? am I missing something?

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